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DNA/RNA labeling
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ADVANTAGES of the FluoresfastTM System |
- The labeling
system can be used with single and double stranded DNA, RNA,
amplicons (nucleic acid amplification products) and
oligonucleotides of different lengths
- No need for
sample purification - the labeling reagent can be directly
added to purified DNA/RNA, PCR reaction mixtures as well as to
crude nucleic acid samples such as cell lysates, total mRNA
preparations and other biological fluids containing DNA or RNA
- Label forms a
Covalently linked Mono-Adduct with a single nucleic acid
strand, hence eliminating the possibility of strand
cross-linking
- One Step
Labeling accomplished in 20 min using a patented Fluorescent
compound (e.g., see MSDS) and a hand-held long wavelength UV
lamp (irradiation at 365 nm).
- Labeling
process DOES NOT require the intervention of any costly
polymerase enzyme or the use of any modified nucleoside
triphosphate substrate
- Labeling
compounds are stable at ambient temperature
- Hybridization
Fidelity fully maintained regardless of the method of
hybridization employed FluoresfastTM labeling system allows
for localized fluorescence emission and does not interfere
with the hybridization function of the DNA
- Labeled
Nucleic Acid Compatible with Microarray Hybridization and
Fluorescence Detection Technology - labeled sample can be
hybridized onto a microarray surface printed with
target-specific immobilized probes and detected using any
commercially available CCD camera and Microarray Scanner
- Multiplexing
Capability: FluoresfastTM Labeling System is capable of
simultaneous analyses of multiple nucleic acid target
sequences from multiple samples through the use of spectrally
distinct signature fluorescent labels attached to the target
nucleic acid sequences and immobilized probes that are
hybridizable to these targets
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