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DNA/RNA labeling  

 
ADVANTAGES of the FluoresfastTM System
  • The labeling system can be used with single and double stranded DNA, RNA, amplicons (nucleic acid amplification products) and oligonucleotides of different lengths
  • No need for sample purification - the labeling reagent can be directly added to purified DNA/RNA, PCR reaction mixtures as well as to crude nucleic acid samples such as cell lysates, total mRNA preparations and other biological fluids containing DNA or RNA
  • Label forms a Covalently linked Mono-Adduct with a single nucleic acid strand, hence eliminating the possibility of strand cross-linking
  • One Step Labeling accomplished in 20 min using a patented Fluorescent compound (e.g., see MSDS) and a hand-held long wavelength UV lamp (irradiation at 365 nm).
  • Labeling process DOES NOT require the intervention of any costly polymerase enzyme or the use of any modified nucleoside triphosphate substrate
  • Labeling compounds are stable at ambient temperature
  • Hybridization Fidelity fully maintained regardless of the method of hybridization employed FluoresfastTM labeling system allows for localized fluorescence emission and does not interfere with the hybridization function of the DNA
  • Labeled Nucleic Acid Compatible with Microarray Hybridization and Fluorescence Detection Technology - labeled sample can be hybridized onto a microarray surface printed with target-specific immobilized probes and detected using any commercially available CCD camera and Microarray Scanner
  • Multiplexing Capability: FluoresfastTM Labeling System is capable of simultaneous analyses of multiple nucleic acid target sequences from multiple samples through the use of spectrally distinct signature fluorescent labels attached to the target nucleic acid sequences and immobilized probes that are hybridizable to these targets